sec16a antibody (Bio-Techne corporation)

Bio-Techne corporation sec16a antibody
Sec16a Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+sec16a/SEC16A+Antibody/bio-techne+corporation___nb100-1799
Average 93 stars, based on 1 article reviews

sec16a antibody - by Bioz Stars, 2026-11

93/100 stars

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rabbit anti-mouse sec16a (Holzel Diagnostika)

Holzel Diagnostika rabbit anti-mouse sec16a

<t>SEC16A</t> forms a complex with PI31 and TBK1. (A) Immunoblot analysis representing immunoprecipitation of endogenous PI31 in DCs following MDP/PAM 3 CSK 4 stimulation for LC-MS/MS analysis. (B) List of proteins from LC/MS analysis with highest confidence score and peptide counts. (C) Relative abundance protein for SEC16A. Data represent mean ± s.e.m; ( n = 2) * P < 0.05; one-way ANOVA (Tukey's multiple comparison test). (D) DCs and (E) THP1 cells stimulated with MDP/PAM 3 CSK 4 . Cell lysates were immunoprecipitated with PI31 antibodies and probed with anti-SEC16A. Data are representative of three independent experiments. Densitometric analysis of SEC16A band intensities normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); * P < 0.05 Student's t test. (F,G) Images show confocal analysis of THP-1 cells transfected with GFP-PI31 (green) for 24 h, and stimulated with MDP/PAM 3 CSK 4 for 30 min and stained with antibody to SEC16A (red) and DAPI (blue). Pearson's coefficient for PI31 and SEC16A co-localization in control or MDP/PAM 3 CSK 4 stimulated cells. Data represent means ± s.e.m. ( n = 20), Three independent experiments with at least five images analyzed per experiment); **** P < 0.0001 Student's t -test. (H) THP1 cells were stimulated with MDP/PAM 3 CSK 4 for 30 min. Cell lysates were immunoprecipitated with anti-TBK1 antibodies and probed with anti-SEC16A, anti-TBK1 and anti-TANK antibodies. Data represent three independent experiments. Densitometric analysis of TBK1 band intensity normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); n.s., non-significant. Student's t -test.

Rabbit Anti Mouse Sec16a, supplied by Holzel Diagnostika, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+sec16a/rabbit+anti+mouse+sec16a/pmc06503738-52-103-106
Average 90 stars, based on 1 article reviews

rabbit anti-mouse sec16a - by Bioz Stars, 2026-11

90/100 stars

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rabbit anti- sec16a polyclonal antibody (Cusabio)

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Rabbit anti-Human SEC16A Polyclonal Antibody

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SEC16A forms a complex with PI31 and TBK1. (A) Immunoblot analysis representing immunoprecipitation of endogenous PI31 in DCs following MDP/PAM 3 CSK 4 stimulation for LC-MS/MS analysis. (B) List of proteins from LC/MS analysis with highest confidence score and peptide counts. (C) Relative abundance protein for SEC16A. Data represent mean ± s.e.m; ( n = 2) * P < 0.05; one-way ANOVA (Tukey's multiple comparison test). (D) DCs and (E) THP1 cells stimulated with MDP/PAM 3 CSK 4 . Cell lysates were immunoprecipitated with PI31 antibodies and probed with anti-SEC16A. Data are representative of three independent experiments. Densitometric analysis of SEC16A band intensities normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); * P < 0.05 Student's t test. (F,G) Images show confocal analysis of THP-1 cells transfected with GFP-PI31 (green) for 24 h, and stimulated with MDP/PAM 3 CSK 4 for 30 min and stained with antibody to SEC16A (red) and DAPI (blue). Pearson's coefficient for PI31 and SEC16A co-localization in control or MDP/PAM 3 CSK 4 stimulated cells. Data represent means ± s.e.m. ( n = 20), Three independent experiments with at least five images analyzed per experiment); **** P < 0.0001 Student's t -test. (H) THP1 cells were stimulated with MDP/PAM 3 CSK 4 for 30 min. Cell lysates were immunoprecipitated with anti-TBK1 antibodies and probed with anti-SEC16A, anti-TBK1 and anti-TANK antibodies. Data represent three independent experiments. Densitometric analysis of TBK1 band intensity normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); n.s., non-significant. Student's t -test.

Journal: Frontiers in Immunology

Article Title: NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses

doi: 10.3389/fimmu.2019.00958

Figure Lengend Snippet: SEC16A forms a complex with PI31 and TBK1. (A) Immunoblot analysis representing immunoprecipitation of endogenous PI31 in DCs following MDP/PAM 3 CSK 4 stimulation for LC-MS/MS analysis. (B) List of proteins from LC/MS analysis with highest confidence score and peptide counts. (C) Relative abundance protein for SEC16A. Data represent mean ± s.e.m; ( n = 2) * P < 0.05; one-way ANOVA (Tukey's multiple comparison test). (D) DCs and (E) THP1 cells stimulated with MDP/PAM 3 CSK 4 . Cell lysates were immunoprecipitated with PI31 antibodies and probed with anti-SEC16A. Data are representative of three independent experiments. Densitometric analysis of SEC16A band intensities normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); * P < 0.05 Student's t test. (F,G) Images show confocal analysis of THP-1 cells transfected with GFP-PI31 (green) for 24 h, and stimulated with MDP/PAM 3 CSK 4 for 30 min and stained with antibody to SEC16A (red) and DAPI (blue). Pearson's coefficient for PI31 and SEC16A co-localization in control or MDP/PAM 3 CSK 4 stimulated cells. Data represent means ± s.e.m. ( n = 20), Three independent experiments with at least five images analyzed per experiment); **** P < 0.0001 Student's t -test. (H) THP1 cells were stimulated with MDP/PAM 3 CSK 4 for 30 min. Cell lysates were immunoprecipitated with anti-TBK1 antibodies and probed with anti-SEC16A, anti-TBK1 and anti-TANK antibodies. Data represent three independent experiments. Densitometric analysis of TBK1 band intensity normalized with αctin ( n = 3). Data represent the mean ± s.e.m. ( n = 3); n.s., non-significant. Student's t -test.

Article Snippet: The antibodies used in the study include: rabbit anti-human PI31 (Atlas Antibodies, HPA041122), rabbit anti-mouse PI31 (Abcam, ab187200); rabbit anti-human P38 (9212), rabbit anti-mouse/human actin (12620), anti-human TBK1 (3504), anti-human IRF-3 (11904), rabbit anti-tubulin (5335), mouse anti-human PSMB8/LMP7 (13726), rabbit anti- human PA28α (9643), rabbit anti-human PSMD2 (25430), rabbit anti-human PSMA2 (11864), rabbit anti-human TANK (2141), rabbit anti-phospho-NF-κB p65 (3033), rabbit anti-human IκBα (9242), mouse anti-human phospho-IκBα (9246), rabbit anti-mouse ERp72 (5033), rabbit anti-mouse LAMP1 (3243), rabbit anti-FLAG (14793), rabbit anti-HA (3724), rabbit anti-GFP (2956), anti-mouse (7076), and anti-rabbit (7074) IgG HRP-linked secondary antibodies were all from Cell Signaling; rabbit-anti-human SEC16A (Abcam, ab70722), rabbit anti-mouse SEC16A (Holzel diagnostika, KIAA0310), mouse anti-human PSMA2 (Enzo, BML-PW8105-0100), mouse anti-human PSMA4 (Enzo, BML-PW8120).

Techniques: Western Blot, Immunoprecipitation, Liquid Chromatography with Mass Spectroscopy, Transfection, Staining

PI31, but not SEC16A, mediates NOD2/TLR2-dependent cross-presentation. (A) PI31 expression was downregulated by transfecting BMDCs with control non-targeting and PI31 siRNAs. Immunoblots data represent three independent experiments. (B,C) 48 h post-transfection, BMDCs were incubated with MDP/PAM 3 CSK 4 in presence or absence of sOVA (0, 0.1, and 1 μg/ml) or SIINFEKL peptide for 3 h followed by co-culture with OT-I CD8 + T cells for 72 h. Cross-presentation efficiency of DCs was determined by the percentage of CD8 + CD69 + T cells using FACS. Data represent the mean ± s.e.m; ( B,C , n = 4). Data are from one experiment representative of three or more independent experiments. n.s. = non-significant; **** P < 0.0001; * P < 0.05; one-way ANOVA (Tukey's multiple comparison test) (D) SEC16A expression was downregulated by transfecting BMDCs with control non-targeting and SEC16A siRNAs. Immunoblots data represent three independent experiments. (E,F) 48 h post-transfection, BMDCs were incubated with MDP/PAM 3 CSK 4 with or without sOVA (0, 0.1, and 1 ug/ml) or SIINFEKL peptide for 3 h followed by co-culture with OT-I CD8 + T cells for 72 h. Cross-presentation was measured by calculating the percentage of CD8 − CD69 − T cells using FACS. Data represent mean ± s.e.m; ( E,F , n = 3). Data from one experiment representative of three or more independent experiments. n.s., non-significant; one-way ANOVA (Tukey's multiple comparison test).

Journal: Frontiers in Immunology

Article Title: NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses

doi: 10.3389/fimmu.2019.00958

Figure Lengend Snippet: PI31, but not SEC16A, mediates NOD2/TLR2-dependent cross-presentation. (A) PI31 expression was downregulated by transfecting BMDCs with control non-targeting and PI31 siRNAs. Immunoblots data represent three independent experiments. (B,C) 48 h post-transfection, BMDCs were incubated with MDP/PAM 3 CSK 4 in presence or absence of sOVA (0, 0.1, and 1 μg/ml) or SIINFEKL peptide for 3 h followed by co-culture with OT-I CD8 + T cells for 72 h. Cross-presentation efficiency of DCs was determined by the percentage of CD8 + CD69 + T cells using FACS. Data represent the mean ± s.e.m; ( B,C , n = 4). Data are from one experiment representative of three or more independent experiments. n.s. = non-significant; **** P < 0.0001; * P < 0.05; one-way ANOVA (Tukey's multiple comparison test) (D) SEC16A expression was downregulated by transfecting BMDCs with control non-targeting and SEC16A siRNAs. Immunoblots data represent three independent experiments. (E,F) 48 h post-transfection, BMDCs were incubated with MDP/PAM 3 CSK 4 with or without sOVA (0, 0.1, and 1 ug/ml) or SIINFEKL peptide for 3 h followed by co-culture with OT-I CD8 + T cells for 72 h. Cross-presentation was measured by calculating the percentage of CD8 − CD69 − T cells using FACS. Data represent mean ± s.e.m; ( E,F , n = 3). Data from one experiment representative of three or more independent experiments. n.s., non-significant; one-way ANOVA (Tukey's multiple comparison test).

Techniques: Expressing, Western Blot, Transfection, Incubation, Co-Culture Assay

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